Effect of Glucose Concentrations on the HSA-Hydrochlorothiazide Interaction - Study using Circular Dichroism and Molecular Docking

Authors

  • Marilia Marilia Amável Gomes Soares Rio de Janeiro State University, Brazil
  • Otávio Augusto Chaves Federal Rural University of Rio de Janeiro
  • Dilson Silva Rio de Janeiro State University, Brazil
  • Darí Cesarín-Sobrinho Federal Rural University of Rio de Janeiro
  • CELIA MARTINS CORTEZ Rio de Janeiro State University

Keywords:

Diabetes mellitus, glucose, hydrochlorothiazide, human serum albumin, CD-spectroscopy, molecular docking

Abstract

We used circular dichroism (far and near UV) to study the interaction between HSA and hydrochlorothiazide in the absence and presence of glucose at 37ºC. Experimental data were correlated to theoretical analysis through molecular docking simulations. As it is a antihypertensive drug used in the treatment of diabetic hypertensive patients, the ability of hydrochlorothiazide binds to HSA was verified in normoglycemic (containing glucose 80 mg/dl) and hyperglycemic (containing glucose 320 mg/dl) solutions. Results confirm that the binding ability of HCTZ binds to albumin decreases with the GLU concentration increase. This generates a perturbation on the protein structure and an instability condition, which difficulties the bind of HCTZ to the protein binding pocket. It suggests that the adverse effects of HCTZ in hyperglycemic patients may be related to increased levels of free drug in the bloodstream by the decrease of its bind to albumin.  ?In this way, effective treatment for diabetic patients with HCTZ is linked to adequate control of the glycemic index.

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Author Biographies

Marilia Marilia Amável Gomes Soares, Rio de Janeiro State University, Brazil

Postgraduation in Medical Sciences, Rio de Janeiro State University, Brazil

Otávio Augusto Chaves, Federal Rural University of Rio de Janeiro

Department of Chemistry, Federal Rural University of Rio de Janeiro

Dilson Silva, Rio de Janeiro State University, Brazil

Postgraduation in Medical Sciences, Rio de Janeiro State University, Brazil

Darí Cesarín-Sobrinho, Federal Rural University of Rio de Janeiro

Department of Chemistry, Federal Rural University of Rio de Janeiro, Brazil

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Published

2022-05-09

How to Cite

Marilia Amável Gomes Soares, M. ., Chaves, O. A., Silva, D. ., Cesarín-Sobrinho, D. ., & MARTINS CORTEZ, C. (2022). Effect of Glucose Concentrations on the HSA-Hydrochlorothiazide Interaction - Study using Circular Dichroism and Molecular Docking. Indian Journal of Pharmacy & Drugs Studies, 7–14. Retrieved from https://mansapublishers.com/index.php/ijpds/article/view/3217

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Original Article