Evaluation of polystyrene petri dish-based method for assessing biofilm formation in vitro by Trichosporon spp. and its comparison with test-tube method

Abstract

Background: Microorganisms growing in a biofilm are associated with chronic and recurrent human infections and are highly
resistant to antimicrobial agents. There are various methods to detect biofilm production such as tube method (TM) and tissue
culture plate method followed by microscopic examination by inverted microscope. Objective: This study was conducted to
compare two methods for the detection of biofilms. Methods: In this study, biofilm formation of ten isolates of Trichosporon
spp. by test TM (TTM) and polystyrene petri dish method (PDM) was compared. Results: In the TTM, they were weak biofilm
producers as compared to PDM where they were strong biofilms producers. Conclusion: PDM can be safely used to find out
pattern of biofilm formation by Trichosporon.